Single-cell protein turnover around the cell cycle

robert.tanguay@ibis.ulaval.ca

Notes: Détails de l'inscription: Frais d'inscription: Conférentier: Contact: Eric Paquet, Ph.D. Postdoctoral fellow, Computational Systems Biology Lab Institute of Bioengineering School of Life Sciences Swiss Institute of Technology, Lausanne (Suisse) Long-term live cell imaging in combination with fluorescent reporters, and computational systems biology is a powerful system enabling to study the behavior of thousand of individual cells in real time. Contrary to whole population analyses, single-cell biology enables us to go one step further in understanding how a heterogeneous pool of individual cells behaves in real-time. I am going to present two applications of single-cell computational systems biology to understand protein dynamics around the cell cycle. Within the first application, we monitored both the circadian clock and the cell cycle in thousands of individual mouse fibroblast cells to understand how the two biological processes are interconnected. For the second application, we use a dual fluorescent reporter composed of a fast and slow maturing proteins to understand protein dynamics (production and degradation) in mouse embryonic stem cells. We discovered that, while most proteins accumulate linearly during interphase, dynamics of protein synthesis and degradation around mitosis are more heterogeneous. Together, our results revealed an unanticipated diversity of protein dynamics around the cell cycle and the important role of computational system biology to study these processes at the single-cell level.
Responsable : Dr Robert M. TANGUAY Professeur titulaire Faculté de médecine Médecine-Dép. biologie moléculaire, biochimie médicale et pathologie 418 656-2131 poste 3339
Christian Landry
Gratuit
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